This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA.
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches.
Nanomedicine is defined as the application of nanobiotechnology in clinical medicine, which is currently being used to research the pathomechanism of disease, refine molecular diagnostics, and aid in the discovery, development, and delivery of drugs.
This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides).
This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which... Læs mere
Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, ß-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.
Includes bibliographical references and index.
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation.