This book highlights recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins, as well as the role of several suppressive molecules in immunoregulation.
This comprehensive technical volume's three sections cover the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determining the function of QS signals in vivo, and developing QS disruption strategies.
Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species.
Protein Expression in Mammalian Cells presents a compendium of vital techniques to further our knowledge of mammalian protein expression. Each chapter includes a brief introduction, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.
Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.
Bacterial Molecular Networks provides authoritative descriptions of various experimental and computational methods that enable the characterization and analysis of molecular interaction networks.
Recently, a new understanding of cell-penetrating peptides has emerged. In this book, expert researchers explore the latest information on cell-penetrating peptides (CPPs). They provide insight into the most important and contemporary areas of CPP research.
Chemical library technologies have brought about dramatic changes in the drug discovery process, and, though still evolving, they have become an integral part of ongoing drug discovery research.
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.
This self-contained reference covers the most relevant RNA-based technologies, including ribozymes, RNA apatmers, siRNAs, and mRNA-encoding therapeutic proteins. Innovated nanotechnologies are presented, in addition to manufacturing protocols.
Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response.
Recent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases.