Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated.
Another key addition to Springer's Methods in Molecular Biology series, this volume includes methods for direct biolistic transfection of DNA using ballistic devices such as a gene gun. Lab-ready protocols and expert troubleshooting guarantee reproducibility.
Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants.
This Methods in Molecular Biology(TM) volume offers detailed instructions for the latest methods which are commonly used to study microbial carotenoids, offering materials and reagents lists, reproducible laboratory protocols, troubleshooting tips and pitfalls.
The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal cord injuries.
Vital to academic researchers, the medical field, and especially to biotech and pharmaceutical scientists, metabolomics is a rapidly expanding field that will provide a key link between functional biology (phenotypes) and the inner workings of cells in tissues or whole organisms.
This highly practical volume, with its step-by-step techniques, brings together recent developments in the statistical methodology of evolutionary genomics and the challenges that followed as a result of rapidly improving sequencing technologies.
This Methods in Molecular Biology(TM) book details methods which are now commonly used to study skin stem cells. These include protocols, materials lists, tips and techniques for the isolation, maintenance and characterization of stem cell populations from skin.
This Methods in Molecular Biology(TM) book offers strategies and protocols for studying a large group of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Includes step-by-step protocols, materials lists, tips and more.
Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs.
Zebrafish (Danio rerio) play an integral role in biomedical research, enabling researchers to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy.