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Stem cell research has achieved much, yet still faces many challenges. In this comprehensive handbook, experts provide step-by-step, proven protocols allowing both novices and experienced researchers to make a comparative assessment--and avoid common pitfalls.
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Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds.
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The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal cord injuries.
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The liver governs many critical life functions and has several distinct cell types. This guide to characterizing liver proteome at every level, from global to subcellular and post-transitional, features laboratory-ready protocols and invaluable expert advice.
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This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. It features cutting-edge methods ready for the laboratory.
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This Methods in Molecular Biology(TM) volume covers LC-MS screening for drugs of abuse, as well as targeted measurement of analytes including abused drugs, toxic compounds, and therapeutic agents. Includes step-by-step protocols, tips and troubleshooting notes.
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Focusing on the two broad areas of epigenetics, DNA methylation and chromatin modifications, this book highlights select techniques that have been mainstays in the field. It also covers methods especially relevant to extant discoveries in epigenetics.
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Written in the popular Methods in Molecular Biology(TM) series format, this book presents a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models.
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This book presents an up-to-date view of yeast as a model eukaryote for systems biology studies. It provides practical, easy to use methodologies and includes key tips and vital implementation advice from the experts.
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Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available... Læs mere
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This book presents computational biology methods by focusing on their applications, including primary sequence analysis, protein structure elucidation, transcriptomics and proteomics data analysis, and exploration of protein interaction networks.
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Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.