Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included.
The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas.
Targeted Therapies for Solid Tumors
By providing a comprehensive overview of the current state of critical care ultrasonography, with content contributed by experts in the field, this text will serve... Læs mere
Kidney Development in Renal Pathology
Clinical research in critical care has exploded in the past several years and we now have a much better understanding of how to care for... Læs mere
Currently, there is no overview on the role of mitochondria in pulmonary diseases and this volume focuses on the mitochondrial metabolism, redox signaling, and mechanisms of mitochondrial pathways in lung injury, inflammation, repair and remodeling.
Imaging Coronary Atherosclerosis
Fructose, High Fructose Corn Syrup, Sucrose and Health
This work, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, in the Methods in Molecular Medicine series presents... Læs mere
From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two most common being asthma and chronic obstructive pulmonary disease (COPD).