This self-contained reference covers the most relevant RNA-based technologies, including ribozymes, RNA apatmers, siRNAs, and mRNA-encoding therapeutic proteins. Innovated nanotechnologies are presented, in addition to manufacturing protocols.
Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response.
Recent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases.
The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. Fungi are highly amenable to molecular work, and a few fungal... Læs mere
Opening with components of the neurovascular unit and the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, this book reviews imaging techniques, molecular study methods, and techniques for delivering therapeutic agents across the BBB.
This changing de?nition of disease means that today’s undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not... Læs mere
Structure-Based Drug Discovery presents an array of methods used to generate crystal structures of biological macromolecules, how to leverage the structural information to design novel ligands anew, and how to iteratively optimize hits and convert them to leads.
In this succinct and practical guide, expert contributors cover standard and novel techniques that facilitate the study of stem cell migration. The book emphasizes in vivo technologies and includes protocols on germ, neuronal, and hematopoietic stem cells.
From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace.
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved.
Coupling features of chromatography and mass spectrometry, SELDI is a popular method of biomarker discovery. This volume reviews its current applications, emphasizing study and experimental design, data analysis and interpretation, and assay development.
This book examines vital topics in protein biochemistry and presents methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, as well as variations on classic techniques.