The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping.
“Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology.
Research into nanopores holds out the promise of ultrafast--and cheaper--DNA sequencing. This study sets out the molecule characterization techniques now available in the field, with numerous step-by-step protocols and expert troubleshooting advice.
This Methods in Molecular Biology(TM) volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics.
Conditions such as oxidative stress and hypoxia, which have a generalized impact on the oxygen metabolism, have been implicated in the genesis of kidney disease. This means... Læs mere
This book presents protocols that address the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Coverage includes protocols in digital imaging and image analysis of stained cells and techniques used in cancer and stem cell research.
Through the rapid development of proteomics methods and technologies, an enormous amount of data was created, leading to a wide-spread rethinking of strategy design and data interpretation.
Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues... Læs mere
This volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research.
These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to... Læs mere
In this book expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing novel approaches to the production of food, feed, fuel, fiber and pharmaceuticals.