The liver governs many critical life functions and has several distinct cell types. This guide to characterizing liver proteome at every level, from global to subcellular and post-transitional, features laboratory-ready protocols and invaluable expert advice.
This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. It features cutting-edge methods ready for the laboratory.
This Methods in Molecular Biology(TM) volume covers LC-MS screening for drugs of abuse, as well as targeted measurement of analytes including abused drugs, toxic compounds, and therapeutic agents. Includes step-by-step protocols, tips and troubleshooting notes.
Focusing on the two broad areas of epigenetics, DNA methylation and chromatin modifications, this book highlights select techniques that have been mainstays in the field. It also covers methods especially relevant to extant discoveries in epigenetics.
Written in the popular Methods in Molecular Biology(TM) series format, this book presents a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models.
This book presents an up-to-date view of yeast as a model eukaryote for systems biology studies. It provides practical, easy to use methodologies and includes key tips and vital implementation advice from the experts.
Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available... Læs mere
This book presents computational biology methods by focusing on their applications, including primary sequence analysis, protein structure elucidation, transcriptomics and proteomics data analysis, and exploration of protein interaction networks.
Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.
Exploring the activation and function of components of the MAPK signaling cascades, this volume presents fully updated techniques to study MAPK. Chapters include lists of... Læs mere
The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks.
Detailing current methodology, this book examines important analytical techniques in chiral separation sciences. It includes step-by-step, readily reproducible laboratory protocols and troubleshooting tips.